Slides were stained to detect the expression of PRDX1 (abcam; Anti-PRDX1 antibody ab41906), γ-H2A.X (abcam; Anti-γ-H2A.X phosphorylated S139 antibody; ab2893), Ki 67 (abcam; Anti Ki67 antibody; ab 15580), 53BP1 (abcam; Anti-53BP1 antibody; ab21083) and cleaved caspase 3 (abcam; Anti-cleaved caspase 3 antibody; ab13847) using an HRP/DAB

Tunicamycin induces NSCLCs apoptosis 5311 Int J Clin Exp Med 2018;11(5):5310-5322 many tumor cells [13]. However, the potential mechanisms of Tunicamycin-induced apoptosis

The Active Caspase 3  caspase-3 (1:500; ab13847; Abcam); and then incubated with secondary antibody (1:5,000; ab205718; Abcam) for. 1 h at room temperature. Protein bands were  Abcam), GFI1 (ab21061, 1:1,000, Abcam), MMP-9 (ab38898, 1:1,000, Abcam), PCNA (ab92552, 1:1,000, Abcam), caspase-3 (ab13847, 1:500, Abcam),  nology, #3498), Bax (1:1000, Cell Signaling Technol- ogy, #2772), pro-caspase3 (1:1000, Abcam, #ab13847), cleaved caspase3 (1:1000, Abcam, #ab49822),  10 Feb 2021 (PCNA, AB13847, Abcam, MA) or an IgG that recognizes cleaved caspase-3 (1: 250, AB13847, Abcam, MA), respectively. Rat anti- mouse CD3  ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. 12 Feb 2019 abcam ab16502: RRID:AB_443394 anti phospho-NF-kB p65 abcam ab86299: abcam ab13847; RRID:AB_443014 anti NLRP3 antibody.

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After incubation, slices were washed by PBS and incubated with FITC-labeled IgG (Abcam, ab74290, 1:2000) for 45 min at room temperature. Digital data. Over the years the members of the lab have produced a lot of different data: Sanger sequences, EST libraries, images and so on. Select a category from the list below in order to view or download the data. was used to determine concentrations of lactate (Abcam, Abcam, ab13847), anti-mitochondrial fission protein 1 (Fis1; 1:1000, Abcam, ab71498), anti-mitofusin IRAK1 antibody (1:200, Abcam, ab238, MA, USA) and Caspase-3 antibody (1:150, Abcam, ab13847, MA, USA) were incubated with the sections at 4℃ overnight.

The sections were incubated with primary antibodies anti-MVH (ab13840, Abcam), LC3B (Ab51520, Abcam), CASP9 (Ab202068, Abcam), CASP3 (Ab13847, Abcam), and Stat3 (9139p, Cell signaling technology

I can recommend to review this on the datasheet for further information. Lane 1: Wild-type HAP1 cell lysate + Staurosporine (1μM for 4h) Lane 2: Wild-type HAP1 cell lysate Lane 3: Caspase-3 knockout HAP1 cell lysate + Staurosporine (1μM for 4h) Lane 4: Caspase-3 knockout HAP1 cell lysate Lanes 1 - 4: Merged signal (red and green). Green - ab184787 observed at 35 kDa.

19 Apr 2018 Primary antibodies included anti-Bcl-2 (ab59348, 1:1000; Abcam, UK), 1:500; Abcam), anti-cleaved-caspase-3 (ab13847, 1:500; Abcam), 

Red - loading control, ab8245, observed at 37 kDa. ab184787 was shown to recognise pro Caspase 3 ab19857 staining Oct4 in F9 cells treated with trans-retinoic acid The cells were incubated at 37°C for 2 days in media containing different concentrations of ab120728 (trans-retinoic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE.

The Active Caspase 3  caspase-3 (1:500; ab13847; Abcam); and then incubated with secondary antibody (1:5,000; ab205718; Abcam) for. 1 h at room temperature. Protein bands were  Abcam), GFI1 (ab21061, 1:1,000, Abcam), MMP-9 (ab38898, 1:1,000, Abcam), PCNA (ab92552, 1:1,000, Abcam), caspase-3 (ab13847, 1:500, Abcam),  nology, #3498), Bax (1:1000, Cell Signaling Technol- ogy, #2772), pro-caspase3 (1:1000, Abcam, #ab13847), cleaved caspase3 (1:1000, Abcam, #ab49822),  10 Feb 2021 (PCNA, AB13847, Abcam, MA) or an IgG that recognizes cleaved caspase-3 (1: 250, AB13847, Abcam, MA), respectively. Rat anti- mouse CD3  ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. 12 Feb 2019 abcam ab16502: RRID:AB_443394 anti phospho-NF-kB p65 abcam ab86299: abcam ab13847; RRID:AB_443014 anti NLRP3 antibody. Recombinant human Cleaved Caspase-3 protein Full length protein datasheet ( ab52314).
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2020-01-10 · Background Increasing evidence has suggested that microRNAs (miRNAs) act as key post-transcriptional regulators in tumor progression. Previous studies have confirmed that miR-17-5p functions as an oncogene in multiple cancers and contributes to tumor progression. However, the role and biological functions of miR-17-5p in the development of laryngeal squamous cell carcinoma (LSCC) still remain antibodies were used: anti-Sox2 (1:500, Abcam 137385); anti-DCX (1:500, Abcam ab18723); anti-β-III tubulin (1:1000, Abcam ab41489); anti-MAP2 (1:500, Sigma Alrich m1406); anti-Ki67 (1:500, Abcam ab15580); anti-Caspase3 (1:500, Abcam ab13847). GFAP-positive as-trocytes were not quantified following detachment and injection, be- 1652 DUAN et al: OBOVATOL INHIBITS TSCC VIA THE EGF-MEDIATED JAK-STAT PATHWAY protein activation, activated caspase 9/3 and apoptosis regu-lator Bax, and that it attenuated the expression of cyclin D1 in Anti-Caspase-8 antibody (ab25901) from Abcam. Manufacturer.

1 h at room temperature.
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ab13847), cleaved caspase 3 (Abcam, ab2302), caspase 9 and cleaved caspase 9 (Abcam, ab184786), IκBα (Cell Signaling Technology, #4814), phosphor‐SPAK/JNK (Cell Signaling Technology, #9255),

(Abcam, ab13847, Cambridge, UK) were assessed im- munohistochemically in other brain hemispheres  15 Nov 2018 ab15580); anti-Caspase3 (1:500, Abcam ab13847). GFAP-positive as- trocytes were not quantified following detachment and injection, be-. 23 Apr 2018 following primary antibodies: NeuN (Millipore, MAB377, 1:300), Epac2 (1:200, Cell Signaling, 43239), caspase-3 (1:200, Abcam, ab13847). Hybridoma Bank) and anti-activated caspase-3 (1:500; Abcam, ab13847). primary anti-Rad51 (1:500; Abcam, ab109107) antibody added overnight.

Caspase3 Abcam ab13847 mmp-2 Abcam ab37150 mmp-9 Abcam ab38898 Table S5. Specific siRNAs for depletion or overexpression of GAS5, YBX1, and miR-21, and nonspecific

Citations for ab13847 by Abcam 781 publications reference this Antibody We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far. Abcam catalog: ab13847 total number of products: 19. domestic rabbit polyclonal reactivity: human, mouse, rat, Xenopus laevis application: western blot 产品报价：询价 更新时间：2013/6/20 13:23:01 产地：美国. 品牌：Abcam.

ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C.